Creative Bioarray Supplies Human Primary Cells for Your Research

Whatever biological research you are focusing on, choosing the right and quality primary cells as the basis for your experiments is a extraordinarily essential and vital preparation process to ensure your successes in the lab.

That’s why Creative Bioarray offers such a wide variety of high quality human primary cells designed to meet your and specific requirements, reduce your time and cost by integrating into your experiments procedures, and enable you an access to rare or difficult to isolate cell types.

Human Primary cell

Primary cells are cells taken directly from a living organism (e.g. biopsy material), and established for growth in vitro. They are not immortalized. Moreover, because of the particular trait, that is going through very few population doublings, primary cells becomes more representative of the main functional component of the tissue from which they are derived, comparing with continuous (tumor or artificially immortalized) cell lines. It also makes primary cells represent a more representative model to the in vivo state, and most biologically and physiologically relevant in vitro tool. 

Primary cells from different species may be used, including human primary cells, and animal primary cells, which allows you to highlight potential differences between humans and preclinical test species.

It is also easily understood that human primary cells are cells isolated from living tissues, such as bone marrow, peripheral blood, cord blood, etc, by using either positive or negative selection techniques. Human primary cells can be used to detect accuracy of extrapolating human data from an animal model.

Use Human Primary Cells from Creative Bioarray

Although being familiar with the significance of high-quality primary cells, most biological researchers however have limited access to use cell lines or isolating primary cells in-house for experimentation.

  

Thus, Creative Bioarray  offers 35 human cell systems with over 160 different cell types, including Adipose Cells, Bone Cells, Endocrine Cells, Hepatic Cells, Nervous Cells, and so many on, to meet your research demands.

The tissue used for the isolation of these cells is obtained from approved medical centers following strict ethical standards. These human primary cells  are extremely accurate as they are literally coming from the source and are available from many sources on the human body. These living samples can give extremely accurate information about the cells in vivo and give relevant information regarding the living systems.

To know more, please contact Creative Bioarray:

Phone: 1-631-626-9181 

Fax: 1-631-614-7828

Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Email: info@creative-bioarray.com

Customizing Your Study by Choosing Tissue Array from Creative Bioarray

Tissue arrays, or tissue microarrays (TMAs), are usually produced by a method of re-locating tissue from conventional histologic paraffin blocks. Through TMAs, researchers get an example of what a tissue microarray and its construction is, as well as knowing what are the completed colon cancer array and spots from this array and a melanoma array.

All of this can be achieved by a hollow needle, which is used to biopsy a standard histologic sections and placing the core (which is as small as 0.6 mm in diameter) into an array on a recipient paraffin block.

As early as 28 years ago, this technique has been described by two people named Wan, Fortuna, and Furmanski, who put forward their ideas in Journal of Immunological Methods. However, the TMAs technique was actually popularized after a publication in Nature Medicine in 1998, written by Kononen and colleagues in the laboratory of Ollie Kallioneimi.

Nowadays, Tissuemicroarrays (TMAs) provide research communities a means for rapid, large-scale analysis of several hundred tissue specimens. 

This High-throughput genome screening technology has also made it possible to survey thousands of genes at a time from tissues and cells. There was no doubting that the translation of such information to improve diagnostic, prognostic and therapeutic applications requires extensive validation.

Creative Bioarray’s tissue arrays help scientists and researchers efficiently analyze gene and protein expressions in multiple tissue samples. We can provide you this high-throughput technique---TMAs, which allows rapid gene expression and copy-number surveys of large numbers of different tissue specimens.

Moreover, based on the availability of more than 500,000 human and 30,000 animal samples in our tissue bank, we have the ability to construct high quality tissue arrays to satisfy your special requirements.

Besides, we have also established standard operation procedure in custom array service. Our tissue microarrays are produced by re-locating tissues from conventional histological blocks. Typically, 40 to 1,000 cylindrical formalin-fixed and paraffin-embedded tissue cores can be densely and precisely arrayed into a single paraffin block. From the block, up to 300 serial 4-8 μm thick sections can be produced and placed on individual glass microscopic slides.

With our professional support and technique, you can have all your required Custom Tissue Array provided by us Creative Bioarray. So, please don't hesitate to call us at 1-631-626-9181 or email to info@creative-bioarray.com for further details.

Custom In Situ Hybridization Service at Creative Bioarray

In situ hybridization (ISH) is a technique for localization and detection of a specific DNA or RNA sequence in whole embryos or tissues/tissue sections/cells using a labeled complementary DNA or RNA strand. DNA and RNA sequences can be visualized after hybridization with labelled probes that are complementary to the sequence of interest.

The underlying basis of In situ hybridization (ISH) is that nucleic acids, if preserved adequately within a histologic specimen, can be detected through the application of a complementary strand of nucleic acid to which a reporter molecule is attached. Visualization of the reporter molecule allows localizing DNA or RNA sequences in a heterogeneous cell population including tissue samples and environmental samples. The technique is particularly useful in neuroscience.

The current applications of this technique include: in situ hybridization to mRNA with oligonucleotide and RNA probes; analysis with light and electron microscopes; whole mount in situ hybridization; double detection of RNAs and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences.

DNA ISH and RNA ISH

DNA ISH can be used to determine the structure of chromosomes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (RNA in situ hybridization) is used to measure and localize RNAs including mRNAs, lncRNAs, and miRNAs, within tissue sections, cells, whole mounts, and circulating tumor cells (CTCs).

Custom ISH Service

Creative Bioarray offers completely customized ISH service from probe design and tissue procurement to expertly interpreted gene expression studies. Its proprietary ISH techniques can dramatically reduce operating costs by eliminating the complicated time-consuming processes of ISH away from laboratory.

When carrying out this technique, cells and tissue sections are typically fixed in 4% paraformaldehyde to preserve morphology for ISH. In some cases, tissues are permeabilized with proteinase K prior to hybridization to improve tissue penetration.

Probes will be prepared by various enzymatic procedures with a reaction mixture that includes labelled nucleotide analogs or radioactive nucleotides, or by direct synthesis as an oligonucleotide. Probes may carry radioactive or fluorescent labels for direct detection or hapten labels for detection by various indirect methods.

Once the sample has been prepared, it is incubated with the probe at elevated temperature to allow the probe to hybridize to the sequence of interest. Unhybridized probe is washed away and the remaining labelled probe is detected.

Please contact us for any special needs in locating special gene on chromosomes or in tissues by using ISH.